I’m black, and I loved Dragon Ball, and back in the late 90s early 2000s when it was airing on Cartoon Network it was pretty much popular with everyone.

I can’t talk for everyone but the saiyan and Frieza arc in Dragon Ball z really stuck with me. Goku knew nothing about his family his people and their history. Then all of a sudden they show up and reck havoc on his life. Eventually he learns that his people were essentially enslaved and exported around the universe as babies to work for their slave owner Frieza, and when they started to organize they were wiped out. This arc ends with a final showdown between the Goku who now knows his history and the last of his race facing down the slave master Frieza who thought he was nothing but a monkey.

Me: Everytime I got time on the confocal

Silicone water bottles take up no space and travel well. Extra leg room seats in normal economy are usually narrower than normal seats due to the table and entertainment. Also no unseat storage, but it’s less of a concern because once the flight is in air can just get your bag down.

I’m 190cm tall and 150kg. I just survived an 11hr flight on Turkish air boeing 777, following an 6hr flight on a Airbus a330 in economy on both. The Airbus was more comfortable. But Dramamine is your friend.

Halo

I will always have a special place in my heart for the Kevin timeline because it was my gateway drug to the good stuff. I wound up watching all of Star Trek in chronical order enterprise to Voyager.

Pittsburgh has some of the worst drivers I’ve ever seen. Running red lights and waiting for yield signs to turn green is the norm there. I’ve seen at least 5 accidents caused by a Pittsburgher stopping at the end of an entrance ramp to a highway.

Unfortunately my car was finally sent to the body shop after 7 years dodging people, and 3 months from leaving the city by a lady who decided to make a left turn from the right lane. Luckily I always have a dashcam running, no one was hurt and the police wrote her a ticket. This lady was a high level Karen, and went ballistic when I asked if she was ok after she crashed into me. Best part was when her husband showed up 10mins later and apologized to me for his, “dumbass wife” who just knocked the mirrors off his truck.

I like to think that I’m a better critical thinker than most, but I fell for the initial news story about her being trans or intersex and the fight being unfair. Then I saw the pictures of her over the years and as a kid, and I dug deeper into what actually happened and I honestly feel dirty. I’ve since been unsubbing to a lot YouTubers.

Loll, his sacrifice was not in vain. I’ve still haven’t seen the extended endings because I was satisfied with the original.

As a comic nerd I felt like I had brain damage after seeing origins in theaters. However, I do agree the major problem with the movie is the last act with “Deadpool” and the battle history was awesome. The video game based on the movie is awesome.

Mass effect for video games, Rebuild of Evangelion for movies, and Old Man war for Books. I know only mass effect is a trilogy (Andromeda doesn’t exist) but I feel like all three stayed with me after finishing them, and the character development and plot lines all had satisfying conclusions.

I can think of 20-30 more interesting stories and characters to make a TV show on from the DC universe

Im the one that was paying for Netflix for my family, but the password crack down motivated me to learn how to build a server and go full arrs. They had a good thing going, but now that $26 a month will be used to buy hdds.

I just wanted to see Luke in the role of obi wan or Yoda after decades of Jedi training be a hero and pass on his legacy by training the next generation. But I would have been ok with one heroic lightsaber battle, and a reunion with han, chewy and Leia.

Watching the last Jedi and seeing someone who tossed his blade away because he saw the good in essentially “Space Hitler” try to kill his own nephew because he was having a nightmare so out of character. Then having him overdose on the force and die like a chump, broke me. I left the theater in silence. The last Jedi is also the only star wars movie with out a light saber fight. No blades ever touched.

Pretty good description. Few extra points. -you don’t need to don’t need to do PCR to have enough Nucleic acids to visualize on the gel. Often you use gels to visualize things like purified plasmids. You can also digest your plasmids with enzymes to confirm genomic insert. -these type of gels have many uses outside of direct comparisons, for example you can use the gel purity DNA of a particular size by cutting out the band, melting the gel and using a silica column to pull the DNA out of the melted gel slice. This is super common when you do molecular cloning.

For what is possibly wrong with the top gel.

• the wavy line in some bands indicates uneven current, this can be due to sample composition before loading the gel, like having cellular debris along with your DNA or a mismatch between the buffer used to make the gel and the running buffer.

-the blurred bands can be a lot of things. It might just be inherited to the sample type, for example raw genomic DNA will look like this because you’ll have enzyme that degrade DNA, and a lot of mid replication chromosomes resulting in fragments existing across a spectrum of sizes equally. Additionally the it could also be user error with overloaded well or not enough loading buffer in the sample so it’s not pushed in to gel evenly, or it was run to fast.

Overall the top gel just looks like crap and the bottom gel looks great, but depending on what was run you really don’t know what the gel should look like, if I was testing endonuclease activity the top gel would probably be what I was hoping to get. If was doing PCR on single gene, the bottom one is better.

I’m from Chicago, I lived in Pittsburgh for 7 years. Best pizza in Pittsburgh was Domino’s.

Buying my own Costco membership was the first moment I truly felt like an adult.

That’s probably more of a reason to get her vaccinated. Those topical flea and tick meds are rough. We got our cat vaccinated even though she was 100% indoor because she used to get too sick with topicals and liked to cuddle the dog.

Poor thing, I’m glad he has a loving family now. I once found a golden retriever wandering the forest while hiking in Indiana. She had so many ticks on her we had to take her to an emergency vet (since it was Sunday) to get them off. Luckly she was microchipped and we found her family who has been looking for her for over a week at the time.

If only there was a vaccine for Lyme disease so the poor pupper wouldn’t have had to suffer in the first place